Detection Of Brugia Malayi And Brugia Pahangi Parasites By Biotinlabeled Dna Probes

摘要

Morphologically, the larvae of Brugian parasites are difficult to differentiate by conventional methods. Recently, radioactive labeled DNA probes2\u273 have been developed to distinguish the larvae of these parasites. However, these probes have a short shelf-life and are hazardous to the users. Two oligonucleotide DNA probes have been tested, one is specific for B.malayi and the other specific for B.pahangi. They were each labeled with Biotin in three different ways by using : a one-tailed 30mer biotinylated uridine residues, a two-tailed 30mer biotinylated uridine-thymidine residues. The dot blot assays were tested at various temperatures (30°C-80°C) using different concentrations of parasite DNAs (12.8ng-0.1ng). Our preliminary results indicated that the sensitivity and specificity of the biotinylated DNA probes, with a two-tailed 45mer biotinylated residues, were highly acceptable for field use.